Melan-A peptides and tumor recognition of A2/Melan-A multimer+ CD8+ allorestricted CTL clones. Recognition of Melan-A peptides and tumor cell lines by A2/Melan-A multimer+ CD8+ allorestricted CTL clones was assessed in a ⁵¹Cr release assay as described in Figure 2. In addition to NA8 (HLA-A2+ Melan-A-) and Me 275 (HLA-A2+ Melan-A+), the melanoma line Me 260 (HLA-A2- Melan-A+) was also included.