|
Frequency of
IFN-gamma-producing T cells in CTL lines stimulated with different HLA-A2+ target cells in
ELISPOT assays.a
|
Effector Cell
|
Target
Cell Line Evaluated
|
|
PCI-13
|
PCI-1
|
PCI-30
|
OSC-19
|
HR
|
None
|
|
CTL-DC#1
(W6/32)
|
17
(33)
|
1,000
(5,000)
|
25,000
(>100,000)
|
1,667
(2,778)
|
12,000
|
20,000
|
|
CTL-DC#2
(W6/32)
|
56
(278)
|
179
(313)
|
132
(278)
|
132
(500)
|
1,200
|
1,500
|
|
CTL-DC#3
(W6/32)
|
6
(8)
|
25
(30)
|
10,000
(20,000)
|
2,857
(6,667)
|
NTb
|
3,333
|
aThe
CTL lines were tested in 20 h ELISPOT assays in response to no
target cells or to the HLA-A2+ HNC lines (PCI-13, PCI-1, PCI-30,
OSC-19) or gastric carcinoma (HR) target cell lines listed. The
ratio of CTL:target cells was 10:1 (i.e. 100,000 CTL: 10,000 target
cells) in all assays. The data are reciprocal frequencies (1/X)
of IFN-gamma producing cells. To confirm that IFN-gamma production
resulted from MHC class I-restricted interaction, W6/32 Abs were
added to duplicate assays, and the reciprocal frequency of IFN-gamma
producing cells in the presence of this Ab is shown in parentheses.
bNT, not tested.
|
