PCI-13 overexpresses both the p53 and MDM-2 proteins. Total PCI-13 (or control DC) lysates were separated on reducing 10% SDS-PAGE gels, Western blotted using specific anti-human p53 and MDM-2 mAbs, and imaged on film by chemiluminescence (panel 1). Immunohistochemistry for p53 and MDM-2 was also evaluated for PCI-13 (and control keratinocyte) cultured cells (panel 2). Cells stained with IgG control (panels 2A and 2D), anti-MDM-2 Abs (panels 2B and 2E) and anti-p53 Abs (panels 2C and 2F) are shown at 200X the original magnification.