|
Percentage
of lymphocyte subclasses in PBMCs on days 1 and 7 of culture with
cytokines.
|
PBMC
Culturec
|
CD3+/CD4+a
|
CD3+/CD8+a
|
CD14+b
|
CD3-/CD16+56+a
|
CD3+/CD16+56+a
|
|
Day
1
|
Day
7
|
Day
1
|
Day
7
|
Day
1
|
Day
7
|
Day
1
|
Day
7
|
Day
1
|
Day
7
|
|
IL-2
(10 ng/ml)d
|
48.2
|
62.1
|
17.5
|
21.2
|
11.8
|
12.1
|
8.5
|
7.9
|
8.0
|
8.1
|
|
GM-CSF
(500 ng/ml)
|
56.6
|
71.7
|
18.3
|
22.1
|
12.1
|
28.1
|
8.4
|
0.7
|
6.1
|
20.1
|
|
IL-12
(100 ng/ml)
|
55.0
|
66.3
|
16.5
|
23.5
|
11.7
|
13.3
|
7.5
|
2.0
|
6.9
|
19.0
|
|
IL-2
+ GM-CSF
|
52.5
|
65.0
|
18.4
|
22.1
|
12.4
|
14.9
|
8.0
|
7.4
|
7.1
|
10.0
|
|
IL-2
+ IL-12
|
56.8
|
68.6
|
18.3
|
22.7
|
11.1
|
14.0
|
6.5
|
4.3
|
5.8
|
13.8
|
|
GM-CSF
+ IL-12
|
52.3
|
70.5
|
16.0
|
22.7
|
10.6
|
16.8
|
8.1
|
2.8
|
6.5
|
15.1
|
|
IL-2
+ GM-CSF + IL-12
|
57.1
|
72.6
|
18.1
|
20.0
|
10.4
|
11.9
|
8.5
|
4.6
|
4.5
|
9.3
|
|
IL-2
(100 ng/ml)
|
55.3
|
68.5
|
17.0
|
18.1
|
12.4
|
13.9
|
8.2
|
10.0
|
6.4
|
9.3
|
|
Medium
|
55.3
|
74.7
|
16.5
|
22.1
|
13.1
|
19.7
|
8.2
|
0.2
|
11.0
|
26.7
|
aResults expressed as percent of total monocytes determined
by FACS analysis of forward and side scatter.
bResults expressed as a percentage of total PBMC analysed
by FACS.
cPBMCs were freshly isolated on day 0 and cultured
in medium alone or medium supplemented with cytokines.
dThe final concentration of IL-2 in culture was 10 or 100
ng/ml when used alone and 10 ng/ml when used in combination. IL-12
and GM-CSF were used at 100 and 500 ng/ml, respectively for both
single and combination treatments.
|
