TNF-alpha release assays following stimulation of the CD8+ T cell lines NW923-IVS-1, NW1274-IVS-1 and NW1539-IVS-1 by COS-7 cell transfectants. TNF-alpha release was detected after stimulation with COS-7 cells co-transfected with the pcDNA3.1 expression vector containing NY-ESO-1 cDNA and pcDNA1Amp containing HLA- B51 cDNA. No specific TNF-alpha release was detected after stimulation with COS-7 cells transfected with HLA-A3 and NY-ESO-1 or with the T cell lines alone.