Western blot analysis of samples from melanoma patient NW29. A lysate of 5x10⁶ NW-MEL-38 melanoma cells, which naturally express NY-ESO-1 (22 kDa), was used for the Western blot. Serum (s; dilution 1:250) and urine (u; dilution 1:1) samples from melanoma patient NW29 were tested for the presence of NY-ESO-1 antibody. Serum from a healthy donor (HD) at a dilution of 1:250 served as a negative control.