Study design of post-operative adjuvant therapy of metastatic disease using gp96 derived from the completely resected primary tumor. Primary tumors, inoculated in the foot, were removed from mice by amputation of the foot, and tumors were used as a source of gp96. Mice with subsequent tumor metastasis were treated early, intermediate or late depending on the day of commencement of post-operative treatment. Control animals received either buffer or gp96 derived from liver.