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A large panel of tumor cells suppresses CTL activity in short-term coculture assays1
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Suppression
of CTL lytic ability2
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Tumor
lines
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Meth
A3
|
+
|
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CMS43
|
+
|
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CMS53
|
+
|
|
UV-5117
|
+
|
|
UV-5125
|
-
|
|
10ME
|
+
|
|
10CR
|
+
|
|
63
|
+
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|
A20
|
+
|
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Normal
cells
|
|
B
cell
|
-
|
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PEC4
|
-
|
|
B/c-N
|
-
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1H-2d anti-H-2b CTLs were cocultured
with the indicated tumor lines or normal cells for three hours,
followed by selection of T cells with Thy1.2 antibody and the
MACS cell sorting system. CTLs were immediately tested in a four
hour 51Cr release assay against EL-4 target cells.
2Greater than 25% suppression of CTL lytic ability
is indicated by a "+"; Less than 25% suppression is indicated
by a "-".
3In further analyses, the CTL suppression induced by
these tumor lines was observed to be reversible. Thus, CTLs which
are allowed to recover overnight in the presence of IL-2 regain
95% of their lytic ability (see Fig. 9b).
4Peritoneal exudate cells.
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