|
Resistance
of Meth A tumor cells to lysis by H-2d-specific CTLs1
|
Target Cells
|
%
51Cr release at E:T ratios:
|
|
Exp. 1
|
Exp. 2
|
|
20
|
10
|
5
|
20
|
10
|
5
|
|
BALB/cJ
Con A blasts2
|
68
|
65
|
52
|
69
|
55
|
27
|
|
C57BL/6
Con A blasts2
|
0
|
0
|
1
|
4
|
0
|
0
|
|
C3H-HeN
Con A blasts2
|
12
|
10
|
0
|
20
|
14
|
11
|
|
10ME
|
73
|
62
|
49
|
72
|
51
|
29
|
|
Meth
A ascites
|
12
|
8
|
0
|
0
|
0
|
3
|
|
Meth
A cultured
|
25
|
19
|
9
|
17
|
10
|
7
|
|
CMS4
|
60
|
49
|
37
|
-
|
-
|
-
|
|
CMS5
|
46
|
34
|
23
|
50
|
34
|
20
|
1C57BL/6 mice were challenged intradermally with 2
x 106 live Meth A cells. After tumor rejection, spleen
cells were harvested and cultured with mitomycin C-treated Meth
A cells (responder to stimulator ratio 20:1). On day 6 CTLs were
tested against the indicated targets in 51Cr release
assays. Shown are two representative experiments, of a total of
five.
2Con A blasts was prepared by culturing 2 x 107
whole spleen cells in 10 ml complete RPMI with Concanavalin A
(final concentration 5 µg/ml). Blasts were harvested after
48 hours and 51Cr-labeled for use as target cells in
CTL assays.
|
