mRNA expression pattern of four antigens associated with a cancer related serological response. (A) Northern blot analysis of NY-BR-41, -49, -62 and -85 mRNA. Testis restricted expression was observed for NY-BR-41, -62 and -85, while NY-BR-49 was expressed in 10/16 normal tissues. (B) Real-time RT-PCR analysis of the level of NY-BR-41 mRNA expression (fg of homologous cDNA ± SEM). Results show low level ubiquitous expression. Highest levels of NY-BR-41 mRNA were detected in testis (43.8 ± 0.83 fg) and peripheral blood leukocytes (35.4 ± 2.65 fg), while lower levels (less than 18.0 fg) were detected in the remaining 12 normal tissues. (C) Real-time RT-PCR analysis of mRNA encoding NY-BR-62 showing low level ubiquitous expression (fg of homologous cDNA ± SEM). Highest levels of NY-BR-62 mRNA expression were detected in testis (270.7 ± 7.13 fg ), thymus (88.8 ± 7.89 fg), and colon (20.2 ± 1.40 fg), while low levels (less 6.0 fg) were observed in 11 other normal tissues. (D) Real-time RT-PCR analysis of mRNA encoding NY-BR-85 showing low level ubiquitous expression (fg of homologous cDNA ± SEM). Expression of NY-BR-85 mRNA was detected in thymus (54.5 ± 6.70 fg ), testis (38.8 ± 2.61 fg), colon (15.7 ± 0.38 fg), spleen (15.0 ± 1.04 fg) and peripheral blood leukocytes (12.5 ± 0.72 fg), while lower levels (less 10 fg) were detected in each of the remaining 9 normal tissues.