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Cytofluorometry
analysis demonstrating the specific coating of anti-tumor Fab-HLA-A2/Flu
conjugates on the surface of HLA-A2 negative tumor target cells,
using a FITC-labeled anti-HLA-A2 mAb. (A) CEA positive colon
carcinoma cells LoVo. (B) ErbB-2 positive breast carcinoma cells
SK-BR-3. (C) CD20 positive B lymphoma cells Daudi were incubated
with anti-CEA Fab-HLA-A2, anti-ErbB-2 Fab-HLA-A2 or anti-CD20
Fab-HLA-A2 conjugates respectively, followed by FITC-labeled anti-HLA-A2
(thick solid histograms) or with the latter conjugate alone (grey
shaded histograms).
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