HAGE protein expression in human melanoma cell lines determined by immunofluorescence. Immunofluorescence was observed under a confocal microscope in the nucleus of a HAGE-negative FM-3 cell line transfected with HAGE cDNA (FM-3/HAGE) but not in a HAGE-negative FM-3 cell line transfected with an empty plasmid (FM-3/Ve). Immunofluorescence was also detected under a confocal microscope predominantly in the nucleus of two HAGE-positive melanoma cell lines, FM-6 and FM-82. Cells stained with a rabbit isotype control only showed staining from the DRAQ5 compound specific to the nucleus. Objective magnification: 50x.