HAGE protein expression in ALC/-Ve and ALC/HAGE tumours determined by immunohistochemical staining. Immunohistochemistry demonstrated the in vivo expression of HAGE at the protein level in ALC/HAGE tumours (D), but not in ALC/-Ve tumours (C) excised from DR1/HHDII mice. No non-specific secondary staining was seen in both ALC/-Ve (A) and ALC/HAGE (B) tumours. HAGE expression was found in the cytoplasm and in the nucleus and appeared to be granular. Objective magnification: 20x.