Cancer Immunity - LICR Tetramer Collection

>Human tetramers >Murine tetramers

September 2009

Table 1. Class I MHC/peptide complexes

Human MHC/peptide complexes:

A list tetramers which are tested on reference T cell lines cell before shipping.
B list tetramers which are suitable for research purposes.
C list tetramers which are suitable for special applications.

All complexes, both human and murine, are prepared with human β2 microglobulin unless listed otherwise.

A List Tetramers:

These reagents are tested on reference T cell lines cell before shipping.

HLA	Protein	Position	Peptide ^*	Complex Type ^**
HLA-A*0201	EBV BMLF1	259-267	GLCTLVAML	BSP
	hCMV pp65	495-503	NLVPMVATV	BSP
	HIV RT	896-904	ILKEPVHGV	BSP
	influenza M1	58-66	GILGFVFTL	BSP
	MAGE-10	254-262	GLYDGMEHL	BSP
	MELAN-A / MART-1	26-35	ELAGIGILTV (39)	BSP
	NY-ESO-1	157-165	SLLMWITQC	BSP
	NY-ESO-1	157-165	SLLMWITQA	BSP
	tyrosinase	369-377	YMNGTMSQV	BSP

B List Tetramers:

These reagents are suitable for research purposes.

HLA ^*	Protein	Position	Peptide ^**	Complex Type ^***
HLA-A*0101	influenza NP	44-52	CTELKLSDY	BSP
	MAGE-3	168-176	EVDPIGHLY	BSP
	tyrosinase	146-156	SSDYVIPIGTY	BSP
	tyrosinase	243-251	KCDICTDEY	BSP
HLA-A*0201	AFP	158-166	FMNKFIYEI	BSP
	AFP	325-334	GLSPNLNRFL	BSP
	BK polyomavirus VP1	90-99	STARIPLPNL	BSP
	BK polyomavirus VP1	108-116	LLMWEAVTV	BSP
	B-Raf	597-606	LLTEKSRWSV	BSP
	CAMEL	1-11	MLMAQEALAFL	BSP
	CEA	605-613	YLSGANLNL	BSP
			YLSGADLNL	BSP
			YLSGANLDL	BSP
		691-699	IMIGVLVGV	BSP
		694-702	GVLVGVALI	BSP
			GLLVGVALI	BSP
			GVLVGVALV	BSP
			GLLVGVALV	BSP
	CSPG4 / HMW-MAA	561-569	SLMVILEHT	BSP
		769-777	ILSNLSFPV	BSP
		1063-1071	LLFGSIVAV	BSP
		2238-2246	LILPLLFYL	BSP
	cytokeratin-18	365-373	ALLNIKVKL	BSP
	DCT / TRP-2	180-188	SVYDFFVWL	BSP
	EBV NA-6	284-293	LLDFVRFMGV	BSP
	EBV BMLF1	259-267	GLATLVAML	BSP
	fibromodulin	250-259	YMEHNNVYTV	BSP
	G250 / CA-IX	24-32	QLLLSLLLL	BSP
	G250 / CA-IX	254-262	HLSTAFARV	BSP
	gp100 / Pmel 17	154-162	KTWGQYWQV	BSP
		209-217	ITDQVPFSV	BSP
		209-217	IMDQVPFSV	BSP
		280-288	YLEPGPVTA	BSP
		280-288	YLEPGPVIA	BSP
		457-466	LLDGTATLRL	BSP
		476-485	VLYRYGSFSV	BSP
	hCMV pp65	14-22	VLGPISGHV	BSP
	hepatitis capsid protein	18-27	FLPSDFFPSV	BSP
	HER2 / neu	369-377	KIFGSLAFL	BSP
	HERV	69-77	DLNNFCQKV (44)	BSP
	HERV-K-Mel		MLAVISCAV (40)	BSP
	HPV16 E7	11-20	YMLDLQPETT	BSP
	HPV16 E7	86-93	TLGIVCPI	BSP
	influenza NA	213-221	CVNGSCFTV	BSP
	influenza NA	213-221	CVNGSCFTI	BSP
	JC polyomavirus VP1	36-44	SITEVECFL	BSP
	LAGE-1	86-94	RLLQLHITM	BSP
	LAGE-1	103-111	ELVRRILSR	BSP
	MAGE-3	112-120	KVAELVHFL	BSP
		157-166	SLQLVFGIEL	BSP
		271-279	FLWGPRALV	BSP
	MAGE-4	230-239	GVYDGREHTV	BSP
	MAGE-4	230-239	GVYDGRIHTV	BSP
	MAGE-C2	336-344	ALKDVEERV	BSP
	Mdm2	81-88	LLGDLFGV	BSP
	MELAN-A / MART-1	26-35	EAAGIGILTV	BSP
			AAAGIGILTV	BSP
			ALAGIGILTV	BSP
			VVAGIGILAI	BSP
		27-35	LAGIGILTV	BSP
		27-35	ALGIGILTV	BSP
	MOG	210-218	TLFVIVPVL	BSP
	mouse TERT	545-553	QLLRSFFYI	BSP
		797-806	SLFDFFLHFL	BSP
		981-990	YLQVNSQTV	BSP
	GnT-V (43)		VLPDVFIRC	BSP
	GnT-V (43)		VLPDVFIRCV	BSP
	NPM-1 (45)	183-191	AIQDLCLAV	BSP
AIQDLCVAV	NPM-1 (45)	183-191	BSP
NY-BR-1	158-167	LLSHGAVIEV	BSP
	960-968	SLSKILDTV	BSP
	1365-1373	LLKEKNEEI	BSP
NY-ESO-1	85-96	SRLLEFYLAMPF	BSP
	86-94	RLLEFYLAM	BSP
	108-116	SLAQDAPPL	BSP
	127-135	TVSGNILTI	BSP
	157-165	ALLMWITQC	BSP
		SALMWITQC	BSP
		SLAMWITQC	BSP
		SLLAWITQC	BSP
		SLLMAITQC	BSP
		SLLMWATQC	BSP
		SLLMWIAQC	BSP
		SLLMWITAC	BSP
	157-166	SLLMWITQCF	BSP
	157-167	SLLMWITQCFL	BSP
	157-170	SLLMWITQCFLPV	BSP
	158-166	LLMWITQCF	BSP
	158-167	LLMWITQCFL	BSP
	159-167	LMWITQCFL	BSP
	161-169	WITQCFLPV	BSP
p53	264-272	LLGRNSFEV	BSP
P. aeruginosa probable sulfate transporter	125-133	LAGIGILIV	BSP
PRAME	300-309	ALYVDSLFFL	BSP
proteinase 3	169-177	VLQELNVTV	BSP
RHAMM	165-173	ILSLELMKL	BSP
Rab-38	49-58	KVLHWDPETV	BSP
Rab-38	50-58	VLHWDPETV	BSP
SSX2	41-49	KASEKIFYV	BSP
	41-49	KASEKITYV	BSP
	103-111	RLQGISPKI	BSP
		ALQGISPKI	BSP
		ALQGASPKI	BSP
		ALQGISAKI	BSP
		ALQGISPAI	BSP
SSX4	41-49	KSSEKIVYV	BSP
survivin	96-104	LTLGEFLKL	BSP
survivin	96-104	LMLGEFLKL	BSP
TERT	540-548	ILAKFLHWL	BSP
tyrosinase	1-9	MLLAVLYCL	BSP
tyrosinase	369-377	YMDGTMSQV	BSP
transaldolase	168-176	LLFSFAQAV	BSP
vaccinia virus C16/B22	60-68	CLTEYILWV	BSP
WT1	126-134	RMFPNAPYL	BSP
WT1	235-243	CMTWNQMNL	BSP
HLA-A*0301	influenza M1	27-35	RLEDVFAGK	BSP
HLA-A*0301	influenza NP	265-273	ILRGSVAHK	BSP
HLA-A*2301	influenza M1	58-66	GILGFVFTL	BSP
HLA-A*2402	HER2 / neu	63-71	TYLPTNASL	BSP
	HIV nef	135-142	RYPLTFGW	BSP
	MAGE-4	143-151	NYKRCFPVI	BSP
	NY-ESO-1	158-166	LLMWITQCF	BSP
	NY-ESO-1	162-170	ITQCFLPVF	BSP
	SAGE	715-723	LYATVIHDI	BSP
	TERT	324-332	VYAETKHFL	BSP
	tyrosinase	206-214	AFLPWHRLF	BSP
	WT1	235-243	CMTWNQMNL	BSP
	WT1	417-425	RWPSCQKKF	BSP
HLA-A*3101	influenza M1	58-66	GILGFVFTL	BSP
HLA-A*6801	gp100 / Pmel 17	182-191	HTMEVTVYHR	BSP
HLA-A*6801	MAGE-3	115-123	ELVHFLLLK	BSP
HLA-B*0702	hCMV pp65	417-426	TPRVTGGGAM	BSP
	HIV nef	129-138	TPGPGVRYPL	BSP
	NY-ESO-1	60-72	APRGPHGGAASGL	BSP
		98-109	TPMEAELARRSL	BSP
		98-110	TPMEAELARRSLA	BSP
HLA-B*1302	influenza M1	58-66	GILGFVFTL	BSP
HLA-B*1801	NY-ESO-1	88-96	LEFYLAMPF	BSP
HLA-B*3501	EBV NA-4	488-496	AVLLHEESM	BSP
	gp100 / Pmel 17	630-638	LPHSSSHWL	BSP
	hCMV pp65	123-131	IPSINVHHY	BSP
	HIV Gag-Pol	774-782	NPDIVIYQY	BSP
	MELAN-A / MART-1	26-35	EAAGIGILTV	BSP
			EPAGIGILTV	BSP
			EAAGIGILTY	BSP
			EPAGIGILTY	BSP
	NY-ESO-1	92-100	LAMPFATPM	BSP
		92-104	LAMPFATPMEAEL	BSP
		93-104	AMPFATPMEAEL	BSP
		94-102	MPFATPMEA	BSP
		94-104	MPFATPMEAEL	BSP
		96-104	FATPMEAEL	BSP
		116-123	LPVPGVLL	BSP
	tyrosinase	312-320	LPSSADVEF	BSP
HLA-B*3503	influenza M1	58-66	GILGFVFTL	BSP
	NY-ESO-1	92-100	LAMPFATPM	BSP
		92-104	LAMPFATPMEAEL	BSP
		93-104	AMPFATPMEAEL	BSP
		94-102	MPFATPMEA	BSP
		94-104	MPFATPMEAEL	BSP
HLA-Cw*0304	importin-α2 / karyopherin	204-212	GAVDPLLAL	BSP
	NY-ESO-1	92-100	LAMPFATPM	BSP
		92-104	LAMPFATPMEAEL	BSP
		96-104	FATPMEAEL	BSP
HLA-Cw*0702	influenza M1	58-66	GILGFVFTL	BSP

^* Also available:
- HLA-A*0201 mutants :D227K, T228A, D227K T228A, T233A, A245V
- HLA-A*1101
- HLA-A*3001
- HLA-A*3004
- HLA-B*0801
- HLA-B*2705
- HLA-B*5101
- HLA-Cw*0303
- HLA-Cw*0401
- HLA-Cw*0602
- HLA-Cw*1402

^** Amino acid residues which have been modified with respect to the native sequence are shown in red in peptide analogs.

^*** Abbreviations: BSP, biotinylation sequence peptide (LHHILDAQKMVWNHR) added C-terminally at the α3 domain.

C List Tetramers:

These reagents are suitable for special applications.

HLA	Protein	Position	Peptide ^*	Complex Type ^**
HLA-A*0201 CD8 binding-deficient tetramers (4, 25, 26)	CEA	694-702	GVLVGVALI	D227K T228A
			GLLVGVALI	D227K T228A
			GVLVGVALV	D227K T228A
			GLLVGVALV	D227K T228A
	EBV IE63	259-267	GLCTLVAML	A245V BSP
	EBV IE63	259-267	GLCTLVAML	D227K T228A
	gp100 / Pmel 17	209-217	ITDQVPFSV	D227K T228A
	gp100 / Pmel 17	209-217	IMDQVPFSV	D227K T228A
	hCMV pp65	495-503	NLVPMVATV	A245V BSP
	hCMV pp65	495-503	NLVPMVATV	D227K T228A
	HER2 / neu	369-377	KIFGSLAFL	D227K T228A
	influenza M1	58-66	GILGFVFTL	A245V BSP
	influenza M1	58-66	GILGFVFTL	D227K T228A
	MAGE-10	254-262	GLYDGMEHL	D227K BSP
				T228A BSP
				A245V BSP
				D227K T228A
	Mdm2	81-88	LLGDLFGV	D227K T228A
	MELAN-A / MART-1	26-35	EAAGIGILTV	D227K T228A
			ELAGIGILTV	D227K BSP
				T228A BSP
				A245V BSP
				D227K T228A
	NY-ESO-1	157-165	SLLMWITQC	D227K BSP
				T228A BSP
				A245V BSP
				D227K T228A
			SLLMWITQA	D227K T228A
	p53	264-272	LLGRNSFEV	D227K T228A
	proteinase 3	169-177	VLQELNVTV	D227K T228A
	RHAMM	165-173	ILSLELMKL	D227K T228A
	SSX2	41-49	KASEKIFYV	D227K BSP
				T228A BSP
				A245V BSP
				D227K T228A
			KASEKITYV	D227K T228A
	WT1	126-134	RMFPNAPYL	D227K T228A
HLA-A0201 α1 α2 H-2Kb α3 ^**	DCT / TRP-2	180-188	SVYDFFVWL	BSP mβ2m
	HERV	65-74	SILQDLNNFV (44)	BSP
	influenza M1	58-66	GILGFVFTL	BSP
	MELAN-A / MART-1	26-35	ELAGIGILTV	BSP
	MELAN-A / MART-1	26-35	ELAGIGILIV	BSP
	NY-ESO-1	157-165	SLLMWITQC	BSP
		157-166	SLLMWITQCF	BSP
		157-167	SLLMWITQCFL	BSP
		158-166	LLMWITQCF	BSP
		158-167	LLMWITQCFL	BSP
		159-167	LMWITQCFL	BSP
		161-169	WITQCFLPV	BSP
Reversible HLA-A*0201 (11, 22)	EBV IE63	259-267	GLCTLVAML
	MELAN-A / MART-1	26-35	ELAGIGILTV
	NY-ESO-1	157-165	SLLMWITQA

^* Amino acid residues which have been modified with respect to the native sequence are shown in red in peptide analogs.

^** Abbreviations: BSP, biotinylation sequence peptide (LHHILDAQKMVWNHR) added C-terminally at the α3 domain; mβ2m, mouse microglobulin.

^*** These are chimeric complexes whose heavy chains are in part human and in part murine.

Murine MHC/peptide complexes

HLA	Protein	Position	Peptide ^*	Complex Type ^**
H-2K^d	HLA-A2	194-203	RYLENGKETL	BSP
	HLA-Cw3	194-203	RYLKNGKETL	BSP
				BSP mβ2m
				D227K BSP ^***
	HER2 / neu	63-71	TYLPTNASL	BSP
	influenza HA	204-212	LYQNVGTYV	BSP
	influenza HA	204-212	LYQNVGTYV	BSP mβ2m
	mouse ERK2	136-144	QYIHSANVL	BSP
	mouse ERK2	136-144	QYIHSANVL	BSP mβ2m
	P. berghei CS	252-260	SYIPSAEKI	BSP
			SYIPSAEKI	BSP mβ2m
			SYIPSAEK(ATTO)I	BSP
			SYIPSAEK(ABA)I	BSP
				BSP mβ2m
				D227K BSP ^***
			Dap[Cy5]-YIPSAEK(ABA)I	BSP
			SYILSAEK(ABA)I	BSP
			SYIASAEK(ABA)I	BSP
H-2D^b	GFP	117-125	DTLVNRIEL	BSP
	GFP	118-125	TLVNRIEL	BSP
	gp100 / Pmel 17	25-33	KVPRNQDWL	BSP
	HPV16 E7	49-57	RAHYNIVTF	BSP
	HPV16 L1	165-173	AGVDNRECI	BSP
	influenza NP (1968)	366-374	ASNENMDAM	BSP
	influenza NP (1968)	366-374	ASNENMDAM	D227K BSP ^***
	influenza NP (1976)	366-374	ASNENMETM	BSP
	influenza NP (1976)	366-374	ASNENMETM	D227K BSP ^***
	influenza PA	224-233	SSLENFRAYV	BSP
	influenza PB1	62-70	LSLRNPILV	BSP
	LCMV GPC	33-41	KAVYNFATC	BSP
			KAVYNFATC	BSP mβ2m
			KAVYNFATA	BSP
				D227K BSP ^***
				Q226A D227K BSP ^***
		276-286	SGVENPGGYCL	BSP
		276-286	SGVENPGGYCL	D227K BSP ^***
	LCMV NP	396-404	FQPQNGQFI	BSP
	mouse HY	738-746	KCSRNRQYL	BSP
	mouse HY	738-746	KCSRNRQYL	D227K BSP ^***
	mouse gp100	25-33	EGSRNQDWL	BSP
	mouse Spas-1 H8	244-252	STHVNHLHC	BSP
	NY-ESO-1	86-94	RLLEFYLAM	BSP
H-2D^k	MPyV MT	389-396	RRLGRTLL	BSP mβ2m
H-2D^d	HIV env	309-318	RGPGRAFVTI	BSP
	HIV env	309-318	RGPGRAFVTI	BSP mβ2m
	NY-ESO-1	81-88	RGPESRLL	BSP
H-2K^b	chicken ovalbumin	258-265	SIINFEKL	BSP mβ2m
	DCT / TRP-2	180-188	SVYDFFVWL	BSP
	E. coli β-gal	97-104	DAPIYTNV	BSP
	H4	87-93	VVYAFKR	BSP
	influenza PB1	703-711	SSYRRPVGI	BSP
	LCMV GPC	34-41	AVYNFATC	BSP
	LCMV NP	205-212	YTVKYPNL	BSP
	MHV S	598-605	RCQIFANI	BSP
	NY-ESO-1	87-94	LLEFYLAM	BSP
	SV40 LT	404-411	VVYDFLKC	BSP
H-2L^d	MLV gp70 AH-1	138-147	SPSYVYHQF (41)	BSP
H-2QAI	mouse H2-L / Qdm	3-11	AMAPRTLLL
TL T3b	TL (42)			BSP

^* Amino acid residues which have been modified with respect to the native sequence are shown in red in peptide analogs. Labels in blue: ABA, 4-azidobenzoic acid; ATTO, low molecular weight fluorescent dyes (http://www.attotech.com/) for flow cytometry analysis. Label in magenta: Dap, diamino-propionic acid.

^** Abbreviations: BSP, biotinylation sequence peptide (LHHILDAQKMVWNHR) added C-terminally at the α3 domain; mβ2m, mouse microglobulin.

^*** CD8 binding-deficient tetramers.