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Variations
in SEREX methodology.
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cDNA library source
- Allogeneic tumor
- Testis
- Tumor cell lines
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Vector system
- Filamentous phage and other prokaryotic expression systems
- Baculovirus and other eukaryotic expression systems
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Antibody source
- Allogeneic sera from cancer patients
- Sera from cancer patients after cancer vaccination
- IgM and other lg subclasses
- Oligoclonal or monoclonal human antibody
- Recombinant clonal IgG
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Combined approaches of SEREX with other cloning methods
- RDA/SEREX
- SAGE/SEREX
- differential display/SEREX
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Abbreviations:
RDA, representational difference analysis; SAGE, serial analysis
of gene expression; SEREX, serological expression cloning.
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